When speaking of antisense therapy, specific scientific techniques and processes involved with it include RNA interference, Alternative Splicing and the use of a RNase H-dependant mechanism. First off, one of the techniques used is RNA interference. What occurs in RNAi is that the antisense drug uses a small interfering RNA, known also as siRNA, to target an mRNA sequence. Then, with the siRNA, the cell is able to use a protein complex named RISC to destroy the disease-causing protein. Moving on to the next method, once the antisense drug binds to the target RNA, is activates a cellular enzyme called RNase H. Once this enzyme is activated, it seeks the target mRNA and destroys it, thus inhibiting the cell’s production of the specific protein. Finally, another technique used in antisense therapy, is alternative splicing. Alternative splicing is primarily a process during gene expression which results in a single gene coding for multiple proteins and the alternative splicing of proteins can lead to a production of proteins that are disease-causing. Antisense drugs in this case are designed to control the splicing and to direct it to make one protein instead of another and to produce a protein that is critical for a normal cellular function to correct a genetic defect.